Definitions/Explanation : Sticky end & Transgene

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9.Biotechnology Principals and Process

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Definitions/Explanation : Sticky end & Transgene

Option A

Option B

Option C

Option D

Solution

$(1)$ The single – stranded extension of a $DNA$ fragment that has been cleaved at a specific sequence often by a restriction endonuclease) in a staggered cut such that this single stranded extension is complementary to those of similarly cleaved $DNAs$.

$(2)$ A gene that is taken from one organism and inserted into the germ line of another organism so that it is replicated as part of the genome and present in all the recipients cells. The resulting organism is described as transgenic.

Standard 12

Biology

Match the column – $I$ with column – $II$ with respectto the nomenclature of enzyme Eco RI and select the correct answer from codes given below.

Column $- I$ Column $- II$

$(A)\; E$ $(1)$ $1^{st}$ in order of identificaiton

$(B)\;Co$ $(2)$ Name of genus

$(C)\; R$ $(3)$ Name of species

$(D)\; I$ $(4)$ Name of strain

medium

Using recombinant technology, genes from a donor cell can be transplanted into a bacterium for $DNA$ replication and protein synthesis. The kinds of cells that can be used as a donor in this technology are

medium

Gene of interest was cloned at site Sal I in $pBR322$. The recombinant plasmid will exhibit susceptibility to

medium

Agarose extracted from sea weeds finds use in:

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$I.$ Ori also controls the copy number of thelinked $DNA$.

$II.$ If a foreign $DNA$ ligates at the Bam Hl site oftetracycline resistance gene in the vector $pBR322$, the recombinant plasmid loses the tetracycline resistance due to insertion offoreign $DNA$.

Choose regarding the above statements.

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Column $- I$	Column $- II$
$(A)\; E$	$(1)$ $1^{st}$ in order of identificaiton
$(B)\;Co$	$(2)$ Name of genus
$(C)\; R$	$(3)$ Name of species
$(D)\; I$	$(4)$ Name of strain

Definitions/Explanation : Sticky end & Transgene

Solution

Similar Questions

Using recombinant technology, genes from a donor cell can be transplanted into a bacterium for $DNA$ replication and protein synthesis. The kinds of cells that can be used as a donor in this technology are

Gene of interest was cloned at site Sal I in $pBR322$. The recombinant plasmid will exhibit susceptibility to

Agarose extracted from sea weeds finds use in:

$I.$ Ori also controls the copy number of thelinked $DNA$. $II.$ If a foreign $DNA$ ligates at the Bam Hl site oftetracycline resistance gene in the vector $pBR322$, the recombinant plasmid loses the tetracycline resistance due to insertion offoreign $DNA$. Choose regarding the above statements.

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$I.$ Ori also controls the copy number of thelinked $DNA$.

$II.$ If a foreign $DNA$ ligates at the Bam Hl site oftetracycline resistance gene in the vector $pBR322$, the recombinant plasmid loses the tetracycline resistance due to insertion offoreign $DNA$.

Choose regarding the above statements.